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You are here: Home / Facilities / FACS

FACS

Flow cytometry is a technology that measures and analyzes the morphological and optical characteristics of single cells suspended in a stream of saline fluid. A focused beam of laser light illuminates each moving cell and light is scattered in all directions. Detectors placed forward of the intersection point or side-on (with respect to the laser beam) receive pulses of scattered light that are converted into a form suitable for computer analysis and interpretation. The physical (morphological) profile of a cell can be observed by combining forward light scatter (FSC) and side light scatter (SSC) analysis. The laser can excite fluorophores that have been used to mark various molecules or physiological functions of the cells. The use of fluorophores with different fluorescence characteristics allows simultaneous multiparametric analysis. The combination of scattered and fluorescence generate useful data to analyze heterogeneous cell populations. 

IGB-ABT FACS Core
The IGB flow cytometry is based on Becton Dickinson FACSAria III. This instrument can register up to 70000 events/second and this guarantees a good signal linearity. It is equipped with 3 lasers beam: 488 nm, 561 nm and 633nm that detect 8 different emission signals of fluorescence for a multiparametric analysis. The instrument can contemporarily separate 4 cellular populations by analyzing events and dividing them at a maximum speed of 3000 cells/second. To separate cells it uses a dropping device that guarantees appropriate temperature, sterility and high cell recovery.

The service provides:
1)Technical support in terms of protocol design assistance and data analysis required by the investigators.
2) Phenotypic analysis of mammalian cells:

  •  cell cycle analysis
  •  surface phenotypic cell analysis
  •  intracellular staining of fixed cells
  •  study of cell survival-apoptosis and cell proliferation.

3) Sorting of mouse and human cells heterogeneous single cell populations into homogeneous populations.
4) Sorting of cell lines transfected with fluorescent reporters.

For more information about our FACS facility and reservations visit the website at this address.

Team:
Pasquale Barba
Vincenzo Mercadante

Contacts:
Email: facsfacility@igb.cnr.it
Phone:  +39 081 6132308