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You are here: Home / Facilities / Integrated Microscopy

Integrated Microscopy

In the post-genomic era both light and electron microscopy have acquired a key role in advancing knowledge in the context of various fields of science (biology, development, immunology, genetics, cell and molecular biology). Indeed, modern microscopy techniques (confocal microscopy, live cell imaging, immuno-electron microscopy) allow the study of the static structure of biological systems, a prerequisite for understanding the function, as well as providing the possibility of a dynamic study of biological processes such as signaling, excitability, transport, apoptosis and development.
A fee-for-service integrated microscopy facility was created at IGB to provide:

  • management and use of high complexity equipments;
  • continuous support and guidance for all research groups;
  • technical support to preparation of investigators’ samples, imaging and data analysis;
  • training to undergraduate and PhD students, post-docs, researchers;
  • protocol design assistance;
  • set up and application of new microscopy technologies;
  • collaborations with other CNR Institutes, Universities, or companies (public or private).

The facility is located on the first floor of the Building 3 in the Pietro Castellino Research Area. Equipment and staff are available to everyone within the IGB.

Researchers from other institutes in the area or universities or companies will be equally welcome. The usage of the scientific common resources of the IGB services by outside laboratories must be authorized (see convention by the Statute and the Rules).

Equipment :

   Microscopes:

  • Heerbrugg Wild M3B (stereomicroscope);
  • Zeiss Stemi SV11 (stereomicroscope);
  • Zeiss Axiophot (upright microscope) with digital camera Nikon Coolpix995;
  • Zeiss Axiovert100 (inverted microscope);
  • Leica DM6000B (fully motorized research microscope system for life science) with Leica digital camera DFC 480 RGB and DFC 350FX B/W; Leica DMI Rb (inverted fluorescence microscope) with digital camera DFC 350FX B/W;
  • Leica DMI6000B (inverted fully automated microscope) with digital camera DFC 420 RGB and DFC 360FX B/W;
  • Leica MZ16 FA (fluorescence stereomicroscope);
  • Leica TCS SP2 AOBS (filter-free spectral laser scanning confocal microscope);
  • JEOL JEM-1011 (transmission electron microscope 100kV, high contrast and brightness with optimum resolution).

    Sectioning and speciment preparation:

  • Reichert Jung 2030 (microtome);
  • Zeiss Microm HM 530 (cryostat for routine histology);
  • Leica VT1000 S (vibrating blade microtome for highly sophisticated sectioning applications);
  • Leica Ultracut UCT (ultramicrotome);
  • Leica EM TP (automated routine tissue processor station);
  • EMITECH K950X (turbo evaporator).

    Imaging analysis:

  • Leica FW4000 and LeicaAF6000 (fluorescence workstation);
  • Leica IM1000 (data base);
  • LeicaQWin (image processing and analysis software);
  • LCS (Leica confocal software).

 

Staff & Contacts
Arbucci Salvatore
Phone: +39 081 6132604
email: salvatore.arbucci@igb.cnr.it

Responsible of the Integrated Microscopy Facility

Rosarita Tatè
Phone: +39 081 6132470
email: rosarita.tate@igb.cnr.it

 

Info:
Phone: + 39 081 6132604