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Welcome

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Welcome at the IGB-IBBC flow citometry facility in the CNR Campus In Naples, via Pietro Castellino 111.

The IGB-IBP flow citometry facility  is equipped with:

These instruments allow the simultaneous detection of two physical parameters and up to 8 different fluorochromes and can be used for the following applications:

• Multicolor staining and immunophenotyping on fixed and vital cells, to detect surface and intracellular markers
• Cell cycle analysis
• Determination of S phase by BrDu and EdU staining
• Identification of M phase by the evaluation of histone -H3 phosphorilation
• Intracellular signalling analysis and cytokine production
• Fuorescent recombinant protein expression
• Determination in live cells of mitochondria membrane potential
• Cell sorting in different support up to single cell cloning.

 

Cell cycle analysis

cellcycle

Cell sorting analysis

cellsorting

Phenotype analysis

phenotypeanalysis

Staff:

•  Dr. Luciana D’Apice  (IBP +390816132584)
•  Dr. Pasquale Barba   (IGB +390816132318)

The staff members offer technical support in experimental design, fluorochrome selections and experimental troubleshooting