{"id":2329,"date":"2021-02-24T10:59:09","date_gmt":"2021-02-24T09:59:09","guid":{"rendered":"https:\/\/www.igb.cnr.it\/?page_id=2329"},"modified":"2023-09-20T10:48:07","modified_gmt":"2023-09-20T08:48:07","slug":"dario-acampora","status":"publish","type":"page","link":"https:\/\/www.igb.cnr.it\/index.php\/dario-acampora\/","title":{"rendered":"Dario Acampora"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2329\" class=\"elementor elementor-2329\" data-elementor-settings=\"{&quot;ha_cmc_init_switcher&quot;:&quot;no&quot;}\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d68670a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d68670a\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;_ha_eqh_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-50fe294 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"50fe294\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;_ha_eqh_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-a674062\" data-id=\"a674062\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fa03ee3 elementor-widget elementor-widget-bdt-member\" data-id=\"fa03ee3\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;_animation&quot;:&quot;none&quot;}\" data-widget_type=\"bdt-member.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"bdt-member skin-default bdt-transition-toggle\">\n\t\t\t\t\t\t\t<div class=\"bdt-member-photo-wrapper\">\n\t\t\t\t\t\n\t\t\t\t\t<div class=\"bdt-member-photo\">\n\t\t\t\t\t\t<div class=\"\">\n\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"2000\" height=\"2000\" src=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039.jpg\" class=\"attachment-full size-full\" alt=\"\" srcset=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039.jpg 2000w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-300x300.jpg 300w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-1024x1024.jpg 1024w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-150x150.jpg 150w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-768x768.jpg 768w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-1536x1536.jpg 1536w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-700x700.jpg 700w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0039-510x510.jpg 510w\" sizes=\"(max-width: 2000px) 100vw, 2000px\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t<div class=\"bdt-member-content\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-top-column elementor-element elementor-element-3130654\" data-id=\"3130654\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9d37fcf elementor-widget elementor-widget-heading\" data-id=\"9d37fcf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Dario Acampora<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-93ba480 elementor-widget elementor-widget-text-editor\" data-id=\"93ba480\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><strong><span style=\"color: #006293;\">Research Director<\/span><\/strong><\/h3><div><div><p><strong>\u00a0<img decoding=\"async\" class=\"alignnone wp-image-2318\" src=\"http:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona.png\" alt=\"\" width=\"17\" height=\"17\" srcset=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona.png 512w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-300x300.png 300w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-150x150.png 150w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-270x250.png 270w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-510x510.png 510w\" sizes=\"(max-width: 17px) 100vw, 17px\" \/> +39 081 6132 443\/441 \u00a0 <img decoding=\"async\" class=\"alignnone wp-image-2317\" src=\"http:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona.png\" alt=\"\" width=\"19\" height=\"19\" srcset=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona.png 512w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-300x300.png 300w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-150x150.png 150w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-270x250.png 270w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-510x510.png 510w\" sizes=\"(max-width: 19px) 100vw, 19px\" \/> dario.acampora@igb.cnr.it<\/strong><\/p><p><strong><span class=\"cretive-button-text\">Embryonic Development and Biology of Stem Cells &#8211; <\/span><\/strong><strong><span class=\"cretive-button-text\">Neurobiology and Brain Functioning<\/span><\/strong><\/p><p><strong>Keywords:\u00a0<\/strong>Parkinson\u2019s disease, mouse modeling, Embryonic stem cells<\/p><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bea5765 e-grid-align-left elementor-shape-rounded elementor-grid-0 elementor-widget elementor-widget-social-icons\" data-id=\"bea5765\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"social-icons.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-social-icons-wrapper elementor-grid\">\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon- elementor-animation-grow-rotate elementor-repeater-item-021f1e1\" href=\"https:\/\/scholar.google.com\/citations?user=i_X24PoAAAAJ&#038;hl=it\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\"><\/span>\n\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" fill=\"#000000\" viewBox=\"0 0 50 50\" width=\"100px\" height=\"100px\"><path d=\"M 25 2 C 12.309534 2 2 12.309534 2 25 C 2 37.690466 12.309534 48 25 48 C 37.690466 48 48 37.690466 48 25 C 48 12.309534 37.690466 2 25 2 z M 25 4 C 36.609534 4 46 13.390466 46 25 C 46 36.609534 36.609534 46 25 46 C 13.390466 46 4 36.609534 4 25 C 4 13.390466 13.390466 4 25 4 z M 21 11 L 11 20 L 17.78125 20 C 17.80125 22.847 19.967531 25.730469 23.769531 25.730469 C 24.129531 25.730469 24.529688 25.690391 24.929688 25.650391 C 24.749688 26.100391 24.560547 26.470078 24.560547 27.080078 C 24.560547 28.230078 25.140391 28.920078 25.650391 29.580078 C 24.020391 29.690078 20.989766 29.879531 18.759766 31.269531 C 16.629766 32.559531 15.980469 34.43 15.980469 35.75 C 15.980469 38.47 18.500469 41 23.730469 41 C 29.930469 41 33.220703 37.510547 33.220703 34.060547 C 33.220703 31.530547 31.779453 30.279922 30.189453 28.919922 L 28.900391 27.890625 C 28.500391 27.570625 27.949219 27.120312 27.949219 26.320312 C 27.949219 25.510313 28.500703 24.989766 28.970703 24.509766 C 30.480703 23.309766 32 21.960234 32 19.240234 C 32 18.197234 31.756203 17.348391 31.408203 16.650391 L 35 13.570312 L 35 17.277344 C 34.405 17.623344 34 18.261 34 19 L 34 25 C 34 26.104 34.896 27 36 27 C 37.104 27 38 26.104 38 25 L 38 19 C 38 18.262 37.595 17.624344 37 17.277344 L 37 12 C 37 11.957 36.980609 11.920906 36.974609 11.878906 L 38 11 L 21 11 z M 24.269531 14.240234 C 27.269531 14.240234 28.820312 18.35 28.820312 21 C 28.820312 21.65 28.739922 22.819922 27.919922 23.669922 C 27.339922 24.259922 26.370938 24.699219 25.460938 24.699219 C 22.370938 24.699219 20.949219 20.620156 20.949219 18.160156 C 20.949219 17.210156 21.14 16.220938 21.75 15.460938 C 22.33 14.710938 23.339531 14.240234 24.269531 14.240234 z M 26.039062 30.609375 C 26.409063 30.609375 26.590859 30.610391 26.880859 30.650391 C 29.620859 32.630391 30.800781 33.620234 30.800781 35.490234 C 30.800781 37.760234 28.97 39.460938 25.5 39.460938 C 21.64 39.460938 19.160156 37.590469 19.160156 34.980469 C 19.160156 32.370469 21.459766 31.499219 22.259766 31.199219 C 23.769766 30.679219 25.719062 30.609375 26.039062 30.609375 z\"><\/path><\/svg>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-06ab9df elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"06ab9df\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;_ha_eqh_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-868f5fb\" data-id=\"868f5fb\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bfdcf77 elementor-widget elementor-widget-eael-adv-tabs\" data-id=\"bfdcf77\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-adv-tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t        <div data-scroll-on-click=\"no\" data-scroll-speed=\"300\" id=\"eael-advance-tabs-bfdcf77\" class=\"eael-advance-tabs eael-tabs-horizontal eael-tab-auto-active \" data-tabid=\"bfdcf77\">\n            <div class=\"eael-tabs-nav\">\n                <ul class=\"\" role=\"tablist\">\n                                            <li id=\"research-interest\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"research-interest-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Research Interest<\/span>                                                    <\/li>\n                                            <li id=\"selected-publications\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"selected-publications-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Selected Publications<\/span>                                                    <\/li>\n                                            <li id=\"professional-experience\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"professional-experience-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Professional Experience<\/span>                                                    <\/li>\n                                    <\/ul>\n            <\/div>\n            \n            <div class=\"eael-tabs-content\">\n\t\t        \n                    <div id=\"research-interest-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"research-interest-tab\">\n\t\t\t\t        <p><strong>Focus. <\/strong>The research focus in our lab is on the genetic modeling of Parkinson\u2019s disease in the mouse.<\/p><p><strong>Research. <\/strong>Over the past, our laboratory has contributed to the understanding of molecular mechanisms that underlie normal and pathological brain development by employing the Otx genes and transgenic mice as genetic model systems. These genes embryonically govern proper specification and patterning of the brain areas, and their altered expression in adulthood can cause neurological and sense organ diseases. Importantly, we discovered that Otx2 is a crucial genetic determinant of a subset of dopaminergic neurons of the <em>ventral tegmental area<\/em> (VTA), which are also characterised by resistance to spontaneous and induced neurodegeneration. Intriguingly, Otx2 is sufficient to antogonise molecular and functional features of other dopaminergic neuron subtypes, including those of the <em>substantia nigra pars compacta<\/em> (SNpc), for which its forced expression can provide protection from induced neurodegeneration.<\/p><p>More recently, through mutant models, both <em>in vivo<\/em> and <em>in vitro<\/em>, we unveiled new Otx2 functions,\u00a0 within the gene network controlling different states of cellular pluripotency and exit from it.\u00a0 Among the most striking ones is the Otx2 dependent cell fate choice leading to the segregation of germ line from soma.<\/p><p><strong>Research project. <\/strong>Over the last couple of years our research has moved back to neuroscience and Parkinson\u2019s disease (PD) in particular. PD is characterized by the loss of dopaminergic neurons of the SNpc associated with high genetic and phenotypic heterogeneity. Thanks to a collaboration with an IGB geneticist (Dr. T. Esposito), a large number of disrupting variants in 26 candidate genes associated with late onset PD (LOPD) has recently been identified. Of note, the co-inheritance of multiple rare variants (\u2265 2) in these 26 genes could predict PD occurrence in about 20% of patients, with high specificity. In order to validate a causal relationship between mutation(s) and disease and with the intent to model PD complexity, we have generated a series of humanized transgenic mouse lines replicating some of the genetic mutations found in patients, also in combination. These lines will undergo molecular, cellular and behavioural investigation for both spontaneous (with aging) and induced neurodegeneration and may represent prospective tools of preclinical relevance.<\/p>                    <\/div>\n\t\t        \n                    <div id=\"selected-publications-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"selected-publications-tab\">\n\t\t\t\t        <p>&#8211; Gialluisi, A., Reccia, M. G., Modugno, N., Nutile, T., Lombardi, A., Di Giovannantonio, L. G., Pietracupa, S., Ruggiero, D., Scala, S., Gambardella, S., International Parkinson\u2019s Disease Genomics Consortium (IPDGC), Iacoviello, L., Gianfrancesco, F., Acampora, D., D&#8217;Esposito, M., Simeone, A., Ciullo, M., &amp; Esposito, T.<\/p><p>Identification of sixteen novel candidate genes for late onset Parkinson&#8217;s disease. <em>Molecular neurodegeneration<\/em>, <em>16<\/em>(1), 35. <a href=\"https:\/\/doi.org\/10.1186\/s13024-021-00455-2\">https:\/\/doi.org\/10.1186\/s13024-021-00455-2<\/a>, (2021).<\/p><p>&#8211; Di Giovannantonio, LG.*, Acampora, D.*, Omodei, D., Nigro, V., Barba, P., Barbieri, E., Chambers, I. and Simeone, A.<\/p><p>Direct repression of Nanog and Oct4 by OTX2 modulates contribution of epiblast-derived cells to germline and somatic lineage. <em>Development<\/em>, <em>148<\/em>(10), <a href=\"https:\/\/doi.org\/10.1242\/dev.199166\">https:\/\/doi.org\/10.1242\/dev.199166<\/a> (2021).<\/p><p>&#8211; Zhang, J., Zhang, M., Acampora, D., Vojtek, M., Yuan, D., Simeone, A., Chambers, I.<\/p><p>OTX2 restricts entry to the mouse germline. <em>Nature<\/em>. 562, 595-599 (2018).<\/p><p>&#8211; Acampora, D., Omodei, D., Petrosino, G., Garofalo, A., Savarese, M., Nigro, V., Di Giovannantonio, L.G., Mercadante, V., Simeone, A.<\/p><p>Loss of the Otx2 binding site in the Nanog promoter affects the integrity of embryonic stem cell subtypes and specification of inner cell mass-derived epiblast. <em>Cell Rep<\/em>. 15<em>, <\/em>2651-2664 (2016).<\/p><p>&#8211; Buecker, C., Srinivasan, R., Wu, Z., Calo, E., Acampora, D., Faial, T., Simeone, A., Tan, M., Swigut, T., Wysocka,J.<\/p><p>Enhancer reorganization in transition from na\u00efve to primed pluripotency. <em>Cell Stem Cell<\/em> 14, 838-853 (2014).<\/p><p>&#8211; Acampora, D., Di Giovannantonio L.G., Simeone, A.<\/p><p>Otx2 is an intrinsic determinant of the Embryonic Stem Cell state and is required for transition to a stable Epiblast Stem Cell condition. <em>Development<\/em>, 140, 43-55 (2013).<\/p><p>&#8211; Di Salvio, M., Di Giovannantonio, LG., Acampora, D., Prosperi, R., Omodei, D., Prakash, N., Wurst, W., Simeone, A.<\/p><p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21057506\">Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.<\/a><\/p><p><em>Nature Neuroscience<\/em>. 13, 1481-1488 (2010).<\/p><p>&#8211;\u00a0 Puelles, E.*, Acampora, D. *, Lacroix, E.*, Signore, M., Annino, A., Tuorto, F., Filosa, S., Corte, G., Wurst, W., Ang, S.L.\u00a0 &amp; Simeone, A.<\/p><p>Otx dose-dependent integrated control of antero-posterior and dorso-ventral patterning of midbrain. <em>Nature Neuroscience <\/em>6, 453-460 (2003).<\/p><p>&#8211; Acampora, D., Pilo Boyl, P., Signore, M., Martinez-Barbera, J.P., Ilengo, C., Puelles, E., Annino, A., Reichert, H., Corte, G. &amp; Simeone, A.<\/p><p>OTD<em>\/<\/em>OTX2 functional equivalence depends on 5\u2019 and 3\u2019 UTR-mediated control of <em>Otx2<\/em> mRNA for nucleo-cytoplasmic export and epiblast-restricted translation. <em>Development<\/em> 128, 4801-4813 (2001).<\/p><p>&#8211; Acampora, D., Postiglione, M.P., Avantaggiato, V., Di Bonito, M., Vaccarino, F., Michaud, J. &amp; Simeone, A.<\/p><p>Progressive impairment of developing neuroendocrine cell lineages in the hypothalamus of mice lacking the <em>Orthopedia<\/em> gene. <em>Genes &amp; Development<\/em> 13, 2787-800 (1999).<\/p><p>&#8211; Acampora, D., Mazan, S., Avantaggiato, V., Barone, P., Tuorto, F., Lallemand, Y., Br\u00fblet, P. &amp; Simeone, A.<\/p><p>Epilepsy and brain abnormalities in mice lacking the <em>Otx1<\/em> gene. <em>Nature Genetics<\/em> 14, 218-222 (1996).<\/p><p>&#8211; Acampora, D., Mazan, S., Lallemand, Y., Avantaggiato, V., Maury, M., Simeone, A. &amp; Br\u00fblet, P.<\/p><p>Forebrain and midbrain regions are deleted in <em>Otx2-\/-<\/em>\u00a0mutants due to a defective anterior neuroectoderm specification during gastrulation. <em>Development<\/em> 121, 3279-3290 (1995).<\/p><p>&#8211; Acampora. D., D&#8217;Esposito, M., Faiella, A., Pannese, M., Migliaccio, E., Morelli, F., Stornaiuolo, E., Nigro, V., Simeone, A. &amp; Boncinelli, E.<\/p><p>The human HOX gene family. <em>Nucl. Acids. Res.<\/em> 17, 10385-10402 (1989).<\/p><p>&#8211; Mavilio, F., Simeone, A., Giampaolo, A., Faiella, A., Zappavigna, V., Acampora, D., Poiano, G., Russo, G., Peschle, C. &amp; Boncinelli, E.<\/p><p>Differential and stage-related expression in embryonic tissue of a new human homeo-box gene.\u00a0 <em>Nature<\/em> 324, 664-668 (1986).<\/p>                    <\/div>\n\t\t        \n                    <div id=\"professional-experience-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"professional-experience-tab\">\n\t\t\t\t        <p><strong>Appointments and scientific career<\/strong><\/p><ul><li>2010 to present: <em>IGB-CNR<\/em>, Research Director (Dirigente di Ricerca)<\/li><li>2006:\u00a0\u00a0 <em>IGB-CNR<\/em>, Senior Research Scientist (Primo Ricercatore)<\/li><li>2001: <em>IGB-CNR<\/em>, Research Scientist (Ricercatore)<\/li><\/ul><ul><li>2000-05: MRC Senior Non-Clinical Fellow at the MRC Centre for Developmental Neurobiology. <em>King\u2019s College London<\/em>, London (UK).<\/li><\/ul><ul><li>2000: Senior Lecturer, Developmental Neurobiology. <em>King\u2019s College London (UK)<\/em>.<\/li><li>1999- 2004: C.N.R. staff scientist, five-year contract at the <em>I.I.G.B<\/em>.-Naples.<\/li><li>1994- 99: C.N.R. staff scientist, five-year contract at the <em>I.I.G.B<\/em>.-Naples.<\/li><li>1993- 94: EMBO long-term fellow. <em>Institut Pasteur<\/em>, Paris (FR).<\/li><\/ul><ul><li>1991- 93: CNR (MISM) research fellow. Project entitled: &#8220;Biotechnologies and Molecular Biology Project&#8221;.<\/li><li>1989- 90: CNR research fellow. Project entitled: &#8220;Mapping and Sequencing of Human Genome Project&#8221;.<\/li><\/ul><ul><li>1988- 89: \u201cFondazione Anna Villa Rusconi\u201d research fellow.<\/li><li>1987:\u00a0\u00a0 Graduated in Biology with honours at the University of Naples. Tesis entitled: \u201dGenomic structure and embryonic expression of two human homeotic genes\u201d.<\/li><\/ul><p>\u00a0<\/p><p><strong>Professional activities<\/strong><\/p><ul><li>2020 to present: Modeling Parkinson\u2019s disease through &#8220;<em>humanized<\/em>\u201d mouse mutants.<\/li><li>2013 to present: Scientific manager of the \u201cEmbryonic Stem Cells and Mouse Modeling (SCMM) facility\u201d at IGB-ABT (http:\/\/mousemodeling.igb.cnr.it\/)<\/li><li>2018-20: Role of Otx2 in Primordial Germ Cells, both <em>in vitro<\/em> and <em>in vivo<\/em>.<\/li><li>2013-17: Roles of Otx2 in the pluripotency regulatory network: identification of target genes through combined analysis of RNA\/ChIP-seq, ChIP and Otx2 binding site mutagenesis both <em>in vitro<\/em> and <em>in vivo.<\/em> Generation and analysis of mutant ESC lines \u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 addressing the Otx2\/Nanog genetic interaction.<\/li><li>2009-12: <em>CEINGE Biotecnologie Avanzate<\/em>, Naples (in partnership with <em>IGB<\/em>)<\/li><\/ul><p>Role of Otx2 gene in mouse Embryonic Stem Cells and their differentiation towards different cell-types. Role of Otx2 gene in epiblast explants and Embryonic Stem Cell-Derived Epiblast Stem Cells (ESD-EpiSC).<\/p><ul><li>2005-09: <em>CEINGE Biotecnologie Avanzate<\/em>, Naples (in partnership with <em>IGB<\/em>)<\/li><\/ul><p>Conditional knock-down and up-regulation of Otx genes expression in mouse models using an inducible system. Focus on dopaminergic progenitors and post-mitotic neurons.<\/p><ul><li>2000-05: <em>KCL, UK<\/em>. Functional studies on the role of the <em>Otx <\/em>genes in midbrain patterning. Functional \u201cevo\/devo\u201d studies on Otx genes through the generation of transgenic mouse models addressing cross-phylum functional equivalence.<\/li><li>1995- 99: Orthopedia (Otp) gene null mice and analysis of Otp function in the neuroendocrine axis. Assessment of Otx1\/Otx2 gene dosage requirement and functional equivalence.<\/li><li>1993- 94: Homologous recombination in ES cells and generation of Otx1 and Otx2 null mice (<em>Institut Pasteur<\/em>. Paris, FR).<\/li><li>1991- 93: Molecular characterization and expression of a new family of brain-specific homeobox containing genes (Otx and Emx).<\/li><li>1985- 90: Isolation and molecular characterization of homeobox containing genes clustered in the HOX1, HOX2, HOX3 and HOX4 loci (genomic collinearity). Human HOX genes in the retinoic acid inducible EC cells system (temporal collinearity).<\/li><\/ul>                    <\/div>\n\t\t                    <\/div>\n        <\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Dario Acampora Research Director \u00a0 +39 081 6132 443\/441 \u00a0 dario.acampora@igb.cnr.it Embryonic Development and Biology of Stem Cells &#8211; Neurobiology and Brain Functioning Keywords:\u00a0Parkinson\u2019s disease, mouse modeling, Embryonic stem cells Research Interest Selected Publications Professional Experience Focus. The research focus in our lab is on the genetic modeling of Parkinson\u2019s disease in the mouse. Research. [&hellip;]<\/p>\n","protected":false},"author":52,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"cybocfi_hide_featured_image":"","footnotes":""},"class_list":["post-2329","page","type-page","status-publish","czr-hentry"],"_links":{"self":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages\/2329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/users\/52"}],"replies":[{"embeddable":true,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/comments?post=2329"}],"version-history":[{"count":111,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages\/2329\/revisions"}],"predecessor-version":[{"id":15926,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages\/2329\/revisions\/15926"}],"wp:attachment":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/media?parent=2329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}