{"id":2354,"date":"2021-02-24T16:06:16","date_gmt":"2021-02-24T15:06:16","guid":{"rendered":"https:\/\/www.igb.cnr.it\/?page_id=2354"},"modified":"2025-01-10T17:44:51","modified_gmt":"2025-01-10T16:44:51","slug":"laura-casalino","status":"publish","type":"page","link":"https:\/\/www.igb.cnr.it\/index.php\/laura-casalino\/","title":{"rendered":"Laura Casalino"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2354\" class=\"elementor elementor-2354\" data-elementor-settings=\"{&quot;ha_cmc_init_switcher&quot;:&quot;no&quot;}\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-536bce31 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"536bce31\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;_ha_eqh_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-5ca2e990\" data-id=\"5ca2e990\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-37103d44 elementor-widget elementor-widget-bdt-member\" data-id=\"37103d44\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;_animation&quot;:&quot;none&quot;}\" data-widget_type=\"bdt-member.bdt-phaedra\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"bdt-member skin-phaedra bdt-transition-toggle\">\n\t\t\t\n\t\t\t\t<div class=\"bdt-member-photo-wrapper\">\n\n\t\t\t\t\t\n\t\t\t\t\t<div class=\"bdt-member-photo\">\n\t\t\t\t\t\t<div class=\"\">\n\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/10\/DSC_0104.jpg\" title=\"DSC_0104\" alt=\"DSC_0104\" loading=\"lazy\" \/>\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t<\/div>\n\n\t\t\t\n\t\t\t<div class=\"bdt-member-overlay bdt-overlay-default bdt-position-cover bdt-transition-fade bdt-position-z-index\">\n\t\t\t\t<div class=\"bdt-member-desc bdt-position-center bdt-text-center\">\n\t\t\t\t\t<div class=\"bdt-member-content bdt-transition-slide-top-small\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-top-column elementor-element elementor-element-47523df9\" data-id=\"47523df9\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6e277b45 elementor-widget elementor-widget-heading\" data-id=\"6e277b45\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Laura Casalino<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3e9c2393 elementor-widget elementor-widget-spacer\" data-id=\"3e9c2393\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4ebc8c71 elementor-widget elementor-widget-text-editor\" data-id=\"4ebc8c71\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><strong><span style=\"color: #006293\">Researcher<br \/><\/span><\/strong><\/h3><div><div><p><strong>\u00a0<img decoding=\"async\" class=\"alignnone wp-image-2318\" src=\"http:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona.png\" alt=\"\" width=\"17\" height=\"17\" srcset=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona.png 512w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-300x300.png 300w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-150x150.png 150w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-270x250.png 270w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/Telefono-icona-510x510.png 510w\" sizes=\"(max-width: 17px) 100vw, 17px\" \/> +39 081 6132 264-463\u00a0 \u00a0 <img decoding=\"async\" class=\"alignnone wp-image-2317\" src=\"http:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona.png\" alt=\"\" width=\"19\" height=\"19\" srcset=\"https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona.png 512w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-300x300.png 300w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-150x150.png 150w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-270x250.png 270w, https:\/\/www.igb.cnr.it\/wp-content\/uploads\/2021\/02\/email-icona-510x510.png 510w\" sizes=\"(max-width: 19px) 100vw, 19px\" \/> laura.casalino@igb.cnr.it<\/strong><\/p><p class=\"elementor-heading-title elementor-size-default\"><span style=\"color: #000000\"><strong><span class=\"cretive-button-text\">Embryonic Development and Biology of Stem Cells<\/span><\/strong><\/span><\/p><p><strong>Keywords: <\/strong>Asplenia, Preclinical models of neurodevelopment <em>(Stem Cells, Primary Cultures, Mice)<\/em>, Non-coding RNAs, Gene expression, Epigenetics<\/p><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c12bf3f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c12bf3f\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;_ha_eqh_enable&quot;:false}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-71a33595\" data-id=\"71a33595\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6439a507 elementor-widget elementor-widget-eael-adv-tabs\" data-id=\"6439a507\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"eael-adv-tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t        <div data-scroll-on-click=\"no\" data-scroll-speed=\"300\" id=\"eael-advance-tabs-6439a507\" class=\"eael-advance-tabs eael-tabs-horizontal eael-tab-auto-active \" data-tabid=\"6439a507\">\n            <div class=\"eael-tabs-nav\">\n                <ul class=\"\" role=\"tablist\">\n                                            <li id=\"research-interest\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"research-interest-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Research Interest<\/span>                                                    <\/li>\n                                            <li id=\"selected-publications\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"selected-publications-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Selected Publications<\/span>                                                    <\/li>\n                                            <li id=\"professional-experience\" class=\"inactive eael-tab-item-trigger eael-tab-nav-item\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"professional-experience-tab\" aria-expanded=\"false\">\n                            \n                            \n                            \n                                                            <span class=\"eael-tab-title title-after-icon\" >Professional Experience<\/span>                                                    <\/li>\n                                    <\/ul>\n            <\/div>\n            \n            <div class=\"eael-tabs-content\">\n\t\t        \n                    <div id=\"research-interest-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"research-interest-tab\">\n\t\t\t\t        <h4><strong><span style=\"color: #003366\">OASI: the Asplenia Observatory for Integrated Studi<\/span>es<\/strong><\/h4><p><strong>A Multidisciplinary Challenge toward an Integrated Approach to Asplenia<\/strong><\/p><p>This collaborative project relies on partnerships among three distinct yet interconnected fields:<\/p><ul><li>Clinics,<\/li><li>biological sciences,<\/li><li>computer science.<\/li><\/ul><p><strong>Background<\/strong><\/p><p><strong>Asplenia<\/strong> arises from either the surgical removal or intrinsic dysfunction of the spleen and is linked to a wide spectrum of hematological, oncological, immunological, and congenital disorders. Patients with asplenia face severe and potentially life-threatening complications, including heightened susceptibility to infections due to immune dysfunction and an increased risk of thrombotic events caused by coagulative and vascular imbalances. These complications profoundly affect long-term health outcomes, leading to greater disability and significant economic burdens on public health systems.<\/p><p>Despite its impact, research on asplenia lacks a unified, systematic approach, largely due to the multiplicity of diseases underlying this condition and to inter-individual variability. Existing studies are often confined to small, disease-specific cohorts, limiting the development of broad, evidence-based clinical guidelines.<\/p><p><strong>The OASI Research Biobank<\/strong><\/p><p><strong>Asplenia<\/strong> represents a multidisciplinary challenge that requires an exhaustive approach to address its complexities effectively. To address these challenges, the OASI Biobank initiative was conceived to provide comprehensive resources for biomedical research on asplenia by collecting, preserving, and analyzing biological samples and related data from asplenic patients.<\/p><p>The <strong>OASI Biobank<\/strong> holds as defining features the transversal approach and the availability of clinical records updated over time. As asplenia occurs in many different clinical contexts, unlike traditional disease-oriented biobanks, the OASI Biobank addresses multiple pathologies intersecting this clinical condition. This innovative perspective enables research into the complex relationships between asplenia and its associated disorders. By broadening our understanding of these intricate interactions, the OASI Biobank aims to improve clinical outcomes and advance personalized medical interventions for asplenic patients.<\/p><p>The OASI Biobank strives to operate on a national scale as a centralized infrastructure through the close collaboration of the <strong>Italian Network for Asplenia (INA)<\/strong>, coordinated by the \u201cLuigi Vanvitelli\u201d University Hospital in Naples. INA is the first and unique clinical network in Italy and Europe dedicated exclusively to the care of asplenic patients. Central to INA\u2019s efforts was establishing an <strong>Observational Clinical Database<\/strong>, which compiles high-quality, prospective, patient-level data.<\/p><p>Through an access-controlled interface with the INA Database, the OASI Biobank incorporates comprehensive clinical data of donors. This seamless integration provides a continuously updated and robust resource that enables the cross-referencing and triangulation of metadata and analytical information from biobank donors with detailed clinical records.<\/p><p>The OASI Research Biobank seeks to facilitate multidisciplinary, multicenter, large-scale integrated studies aimed to fill critical gaps in asplenia biomedical research. Furthermore, OASI will promote bioinformatics innovation by leveraging computational biology to provide insights into asplenia.<\/p><p><strong>Research linked to the OASI Biobank: the ABCare project.<\/strong><\/p><p><u>The ABCare project relies on:<\/u><\/p><p>1) examination of biological material of donors to generate analytical data (<em>at <\/em><em>cellular, sub-cellular, and molecular levels using lab-scale and high-throughput approaches)<\/em>.<\/p><p>2) computational analysis <em>(data integration, <\/em><em>statistical testing of longitudinal data, correlation analysis, and comparative association)<\/em> on:<\/p><ul><li>analytical data (including omics)<\/li><li>metadata<\/li><li>biometric, clinical, and laboratory information <em>(retrospective and prospective data with quantitative and\/or qualitative variables)<\/em><\/li><\/ul><p><u>to perform:<\/u><\/p><p><strong>observational<\/strong> and <strong>experimental<\/strong> <strong>studies<\/strong> intersecting multiple diseases:<\/p><ul><li>cross-sectional research <em>(multiple experimental variables at a given instance analyzed in conjunction with retrospective clinical data)<\/em><\/li><li>longitudinal survey (<em>few experimental variables collected over time analyzed with retrospective and prospective clinical information)<\/em>. Analysis<\/li><\/ul><p><u>with the aim of:<\/u><\/p><p>identifying clinical biomarkers of disease severity and prognostic tools for splenectomy evaluation and asplenia management:<\/p><ul><li>Risk biomarkers <em>&#8211; for the assessment of long-term thrombotic and infectious risk in asplenic patients<\/em>.<\/li><li>Prognostic biomarkers <em>&#8211; to predict the course of asplenia, to assess its severity and progression, regardless of the underlying disease<\/em>.<\/li><li>Screening biomarkers <em>&#8211; for the subclinical assessment of splenic function<\/em>.<\/li><li>Predictive biomarkers <em>&#8211; to develop risk scores to evaluate and prevent criticism of the recognized consequences and complications associated with therapeutic splenectomy.<\/em><\/li><\/ul><p><strong>Funding:<\/strong><\/p><p><strong>\u201cABCare: The Asplenia Biobanking Community: from Analytes to theRapEutic decision making\u201d.<\/strong><\/p><p><strong>MUR PRIN 2022<\/strong>. Macrosettore LS-Life Sciences, Settore ERC LS7 \u201cPrevention, Diagnosis and Treatment of Human Diseases\u201d, Codice progetto 2022Y59MHL_LS7_PRIN2022.<\/p><p><strong>Biobank Governance:<\/strong><\/p><p><em>Biobank Coordinator: Dott.ssa Laura Casalino<br \/>Biobank Investigator: Dott.ssa Marcella Vacca <\/em><\/p><p><strong>External Partners and People:<\/strong><\/p><p>\u201cLuigi Vanvitelli\u201d University Hospital of Naples:<\/p><p style=\"padding-left: 40px\"><em>Prof.ssa Maddalena Casale<\/em><\/p><p>Institute for High-Performance Computing and Networking, ICAR-CNR.<\/p><p style=\"padding-left: 40px\"><em>Dott.ssa Ilaria Granata<\/em><\/p><p style=\"padding-left: 40px\"><em>Dott. Maurizio Giordano<\/em><\/p><p>\u00a0<\/p><h4><strong><span style=\"color: #003366\">Profiling the Mecp2 allelic expression in mouse brain development as a premise for its therapeutic reactivation in RTT<\/span>.<\/strong><\/h4><p>The X-linked <strong>MECP2<\/strong> gene is extensively studied in bridging epigenetics with human health. MECP2-associated diseases suggest that mammalian cells are susceptible to MeCP2 dosage. In females, heterozygous mutations cause <strong>Rett syndrome<\/strong> (RTT, OMIM: 312750), a severe neurodevelopmental disorder, while in males, hemizygous mutations lead to a more severe neonatal encephalopathy. Conversely, proximal Xq28 duplication syndrome (OMIM: 300260 300815), a functional Xq28 disomy involving the MECP2 gene, mainly affects males and results in neurodegeneration. These clinical findings suggest that MeCP2 functions are crucial in the intersection between neurodevelopmental and neurodegenerative disorders.<\/p><p>Moreover, compared to males, females better tolerate the adversities of X chromosome-related genetic diseases due to the neuroprotective action of the Lyon effect. This epigenetic process ensures dosage compensation between genders through X chromosome inactivation (XCI). XCI involves the random silencing of one of the X chromosomes during early embryonic development, with stable epigenetic mechanisms clonally inherited. Due to XCI, Mecp2 prevalently shows monoallelic expression: patients are an epigenetic mosaic of cells expressing either the mutant or the healthy allele, whose proportion in pathogenic tissues correlates with disease severity. However, evidence suggests that the murine Mecp2 may behave as a facultative lineage-specific escape from XCI that can be reactivated through XCI counteracting compounds. Therefore, we established an easy-to-use approach to monitor the spatial and temporal inactivation\/activation status of Mecp2 and distinguish the allelic contribution to the MecP2 protein expression at a single-cell level. We developed female double knock-in mice with a two-color fluorescent reporter system tagging each of the Mecp2 alleles. Among major advantages, this transgenic strain preserves the physiological XCI, thus allowing the tracing of the allelic contribution and the parent-of-origin effect in the expression of MeCP2 protein. Our preliminary evidence suggests that Mecp2 epigenetic mosaicism in the brain might hide elusive details, namely that Mecp2 shows a spatially and developmentally regulated biallelic expression in a restricted portion of brain cells and areas. In perspective, such findings could foster reconsideration of some unsolved issues related to the MECP2-dependent Rett syndrome, while providing new paths to epigenetic manipulations for therapeutic purposes, aimed at the specific reactivation of the MeCP2-locus.<\/p>                    <\/div>\n\t\t        \n                    <div id=\"selected-publications-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"selected-publications-tab\">\n\t\t\t\t        <p>\u00a0<\/p><ol><li>Casalino L, Talotta F, Matino I, Verde P. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/37176013\/\">FRA-1 as a Regulator of EMT and Metastasis in Breast Cancer.<\/a> Int J Mol Sci. 2023 May 5;24(9). doi: 10.3390\/ijms24098307. Review. PubMed PMID: 37176013; PubMed Central PMCID: PMC10179602.<\/li><li>Righetto I, Gasparotto M, Casalino L, Vacca M, Filippini F.\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/36671555\/\">Exogenous Players in Mitochondria-Related CNS Disorders: Viral Pathogens and Unbalanced Microbiota in the Gut-Brain Axis.<\/a> 2023 Jan 13;13(1). doi: 10.3390\/biom13010169. Review. PubMed PMID: 36671555; PubMed Central PMCID: PMC9855674.<\/li><li>Casalino L, Talotta F, Cimmino A, Verde P. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/35326630\/\">The Fra-1\/AP-1 Oncoprotein: From the &#8220;Undruggable&#8221; Transcription Factor to Therapeutic Targeting.<\/a> Cancers (Basel).\u00a02022 Mar 14;14(6). doi: 10.3390\/cancers14061480. Review. PubMed PMID: 35326630; PubMed Central PMCID: PMC8946526.<\/li><li>Casalino L, Verde P. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32806509\/\">Multifaceted Roles of DNA Methylation in Neoplastic Transformation, from Tumor Suppressors to EMT and Metastasis.<\/a> Genes (Basel).\u00a02020 Aug 12;11(8). doi: 10.3390\/genes11080922. Review. PubMed PMID: 32806509; PubMed Central PMCID: PMC7463745.<\/li><li>Talotta F, Casalino L, Verde P. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32385348\/\">The nuclear oncoprotein Fra-1: a transcription factor knocking on therapeutic applications&#8217; door.<\/a> 2020 Jun;39(23):4491-4506.\u00a0doi: 10.1038\/s41388-020-1306-4. Epub 2020 May 8. Review. PubMed PMID: 32385348.<\/li><li>D&#8217;Aniello C, Cermola F, Palamidessi A, Wanderlingh LG, Gagliardi M, Migliaccio A, Varrone F, Casalino L, Matarazzo MR, De Cesare D, Scita G, Patriarca EJ, Minchiotti G.\u00a0<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31061065\/\">Collagen Prolyl Hydroxylation-Dependent Metabolic Perturbation Governs Epigenetic Remodeling and Mesenchymal Transition in Pluripotent and Cancer Cells.<\/a> Cancer Res. 2019 Jul 1;79(13):3235-3250. doi: 10.1158\/0008-5472.CAN-18-2070. Epub 2019 May 6. PubMed PMID: 31061065.<\/li><li>D&#8217;Aniello C, Fico A, Casalino L, Guardiola O, Di Napoli G, Cermola F, De Cesare D, Tat\u00e8 R, Cobellis G, Patriarca EJ, Minchiotti G. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25857264\/\">A novel autoregulatory loop between the Gcn2-Atf4 pathway and (L)-Proline metabolism controls stem cell identity.<\/a> Cell Death Differ.\u00a02015 Jul;22(7):1094-105. doi: 10.1038\/cdd.2015.24. Epub 2015 Apr 10.\u00a0PubMed PMID: 25857264; PubMed Central PMCID: PMC4572871.<\/li><li>Iannone M, Ventre M, Formisano L, Casalino L, Patriarca EJ, Netti PA. <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25699511\/\">Nanoengineered surfaces for focal adhesion guidance trigger mesenchymal stem cell self-organization and tenogenesis.<\/a> Nano Lett. 2015 Mar 11;15(3):1517-25. doi: 10.1021\/nl503737k. Epub 2015 Feb 24.PubMed PMID: 25699511.<\/li><li>Casalino L, D&#8217;Ambra P, Guarracino MR, Irpino A, Maddalena L, Maiorano F, Minchiotti G, Patriarca EJ. Image Analysis and Classification for High-Throughput Screening of Embryonic Stem Cells. In: Zazzu V, Ferraro MB, Guarracino M, editors. Mathematical Models in Biology. Bringing Mathematics to Life; 2014 October 27; Naples, NA, ITALY. DOI 10.1007\/978-3-319-23497-7 Print ISBN 978-3-319-23496-0 Online ISBN 978-3-319-23497-7: Springer International Publishing; c2015.<\/li><\/ol>                    <\/div>\n\t\t        \n                    <div id=\"professional-experience-tab\" class=\"clearfix eael-tab-content-item inactive\" data-title-link=\"professional-experience-tab\">\n\t\t\t\t        <p><strong>Actual position: <\/strong><\/p><p><strong>CNR Research Scientist<\/strong>, since February 2010<\/p><p><strong>Professional Roles:<\/strong><\/p><p><strong>Since Jan 2025: Coordinator of the OASI Research Biobank for Asplenia<\/strong><\/p><p><strong>Since 2020<\/strong>: <strong>Principal Investigator<\/strong><\/p><p>at the IGB-ABT, CNR of Naples<\/p><p><em>Developmental Biology and Genetics<\/em><\/p><p><strong>April 2006 \u2013 2020 Responsible for the Molecular Screening Laboratory<\/strong><\/p><p>\u201cStem Cell Fate Lab\u201d, at the IGB-ABT, CNR of Naples.<\/p><p><em>\u201cAn automated robotic platform designed to identify molecules that control self-renewal, pluripotency, and differentiation of stem cells\u201d.<\/em><\/p><p><strong>April 2006 \u2013 January 2010 Research Fellow<\/strong><\/p><p>Supervisor: Dr. Eduardo J. Patriarca<\/p><p>\u201cStem Cell Fate Lab\u201d, at the IGB-ABT, CNR of Naples.<\/p><p><em>\u201c<\/em><em>The role of L-Pro in metabolic control of mESC metastability and pluripotency.<\/em><em>\u201d<\/em><\/p><p><strong>June 2004 &#8211; March 2006<\/strong>: <strong>Post-doctoral fellow<\/strong><\/p><p>Supervisor: Prof. Moshe Yaniv<\/p><p>Department of Developmental Biology, Pasteur Institute, Paris, France.<\/p><p><em>\u201cFra-1 as a cell cycle sensor and regulator in thyroid neoplastic cells: the cyclin gene A identified as a new transcriptional target of Fra-1\u201d.<\/em><\/p><p><strong>2001-2003: Visiting Fellow<\/strong><\/p><p>Supervisor: Prof. Moshe Yaniv<\/p><p>Department of Developmental Biology, Pasteur Institute, Paris, France.<\/p><p><em>\u201cMolecular mechanisms controlling the proliferation of transformed thyroid cells\u201d.<\/em><\/p><p><strong>2000 &#8211; May 2004: Post-doctoral fellow<\/strong><\/p><p>Supervisor: Dr. Pasquale Verde<\/p><p>Laboratory of Human Genetics, at the IGB-ABT of the CNR in Naples.<\/p><p><em>\u201cThe role of Fra-1 in the control of the cell cycle of thyroid neoplastic cells<\/em>\u201d.<\/p><p><strong>1995 \u20131999: PhD student<\/strong><\/p><p>Supervisor: Dr. Pasquale Verde<\/p><p>Laboratory of Human Genetics, at the IGB-ABT of the CNR in Naples.<\/p><p><em>\u201cPositive and negative transcriptional control mechanisms in the human urokinase gene enhancer region\u201d<\/em><\/p><p><em>\u201cRole of the transcriptional factors AP-1 and NF-kB in the neoplastic transformation of thyroid cells\u201d<\/em><\/p><p><strong>January 1993 &#8211; March 1995: Graduate student<\/strong><\/p><p>Supervisor: Prof. Francesco Blasi<\/p><p>Department of Genetics, University of Milan.<\/p><p><em>\u201cThe transcription factors AP-1 and NF-kB as mediators of the response to tumor promoters and human cytokines of the urokinase gene in lung cancer cells<\/em>\u201d.<\/p>                    <\/div>\n\t\t                    <\/div>\n        <\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Laura Casalino Researcher \u00a0 +39 081 6132 264-463\u00a0 \u00a0 laura.casalino@igb.cnr.it Embryonic Development and Biology of Stem Cells Keywords: Asplenia, Preclinical models of neurodevelopment (Stem Cells, Primary Cultures, Mice), Non-coding RNAs, Gene expression, Epigenetics Research Interest Selected Publications Professional Experience OASI: the Asplenia Observatory for Integrated Studies A Multidisciplinary Challenge toward an Integrated Approach to Asplenia [&hellip;]<\/p>\n","protected":false},"author":73,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"cybocfi_hide_featured_image":"","footnotes":""},"class_list":["post-2354","page","type-page","status-publish","czr-hentry"],"_links":{"self":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages\/2354","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/users\/73"}],"replies":[{"embeddable":true,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/comments?post=2354"}],"version-history":[{"count":64,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages\/2354\/revisions"}],"predecessor-version":[{"id":17738,"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/pages\/2354\/revisions\/17738"}],"wp:attachment":[{"href":"https:\/\/www.igb.cnr.it\/index.php\/wp-json\/wp\/v2\/media?parent=2354"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}