Flow citometry:

Flow cytometry facility management and scientific supervision. The  in-depth knowledge flow citometry  allow me  to support the research groups  in  their approach to flow cytometry. In particular  I collaborate in the experimental design, choice of polychromatic antibody panel, data acquisition and analysis.

Facs Facility LINK

Research topics:

• Study of HLA genes regulation and expression  in autoimmune disease such as celiac disease, diabetes and multiple sclerosis.
• Identification of new biomarkers in immunoopathogenesis of celiac disease and other autoimmune diseases
• Unravelling the pathophysiology of immune tolerance in food allergies and intolerances in collaboration with the "Immunonutrition lab" at Federico II University.
• Analysis of PA2G4 pseudogene 4 (PA2G4P4): identification of sequence and functions in bladder carcinoma and glioblastoma.

Farina F. ,Pisapia L., Laezza M., Serena G., Rispo A., Ricciolino S., Gianfrani C., Fasano A., Del Pozzo, G.

Effect of Gliadin Stimulation on HLA-DQ2 . 5 Gene Expression in Macrophages from Adult Celiac Disease Patients. Biomedicines  2022, 10 (1):63

https://doi.org/10.3390/biomedicines10010063

Paparo L, Nocerino R, Ciaglia E, Di Scala C, De Caro C, Russo R, Trinchese G, Aitoro R, Amoroso A, Bruno C, Di Costanzo M, Passariello A, Messina F, Agangi A, Napolitano M, Voto L, Gatta GD, Pisapia L, Montella F, Mollica MP, Calignano A, Puca A, Berni Canani R.

Butyrate as a bioactive human milk protective component against food allergy.

Allergy. 2021 May;76(5):1398-1415. doi: 10.1111/all.14625. Epub 2020 Nov 16.

PMID: 33043467

https://pubmed.ncbi.nlm.nih.gov/33043467/

Paparo L, Picariello G, Bruno C, Pisapia L, Canale V, Sarracino A, Nocerino R, Carucci L, Cosenza L, Cozzolino T, Berni Canani R.

Tolerogenic Effect Elicited by Protein Fraction Derived From Different Formulas for Dietary Treatment of Cow's Milk Allergy in Human Cells.

Front Immunol. 2021 Feb 12;11:604075. doi: 10.3389/fimmu.2020.604075. eCollection 2020.

PMID: 33679694

https://pubmed.ncbi.nlm.nih.gov/33679694/

Pisapia L., Picascia S., Farina F., Barba P., Gianfrani C., Del Pozzo G., Differential expression of predisposing HLA-DQ2.5 alleles in DR5/DR7 celiac disease patients affects the pathological immune response to gluten. Scientific Reports 2020 Oct 14;10(1):17227.

doi: 10.1038/s41598-020-73907-2.

https://pubmed.ncbi.nlm.nih.gov/33057065/

Pisapia L, Terreri S, Barba P, Mastroianni M, Donnini M, Mercadante V, Palmieri A, Verze P, Mirone V, Altieri V, Califano G, Liguori GL, Strazzullo M, Cimmino A, Del Pozzo G

Role of PA2G4P4 pseudogene in bladder cancer tumorigenesis

Biology, 2020 Mar 31;9(4):66. doi: 10.3390/biology9040066.

https://pubmed.ncbi.nlm.nih.gov/32244410/

Paparo L,Tripodi L, Bruno C, Pisapia L, Damiano C, Pastore L, Berni Canani R

Protective action of Bacillus clausii probiotic strains in an in vitro model of Rotavirus infection

 Sci Rep, 2020 Jul 28;10(1):12636. doi: 10.1038/s41598-020-69533-7

https://pubmed.ncbi.nlm.nih.gov/32724066/

The HLA-DRB1 risk alleles for multiple sclerosis are differentially expressed in blood cells of patients from Southern Italy.

Pisapia L, Cerillo I, Farina F, Zimbardo A, Barba P, Orefice G, Gianfrani C, Strazzullo M, Del Pozzo G.Int J Immunogenet. 2019 Dec;46(6):479-484. doi: 10.1111/iji.12450. Epub 2019 Jul 17.PMID: 31313885

https://pubmed.ncbi.nlm.nih.gov/31313885/

Tristetraprolin/ZFP36 Regulates the Turnover of Autoimmune-Associated HLA-DQ mRNAs.

Pisapia L, Hamilton RS, Farina F, D'Agostino V, Barba P, Strazzullo M, Provenzani A, Gianfrani C, Del Pozzo G.Cells. 2019 Dec 4;8(12):1570. doi: 10.3390/cells8121570.PMID: 31817224

https://pubmed.ncbi.nlm.nih.gov/31817224/

Farina F., Picascia S., Pisapia L., Barba P., Vitale S., Franzese A., Mozzillo E., Gianfrani C., Del Pozzo    G., HLA-DQA1 and HLA-DQB1 Alleles, Conferring Susceptibility to Celiac Disease and Type 1 Diabetes, are    More Expressed Than Non-Predisposing Alleles and are Coordinately Regulated. Cells 2019 Jul 19;8(7):751.

 doi: 10.3390/cells8070751.

https://www.ncbi.nlm.nih.gov/pubmed/31331105

Pisapia L., Camarca A., Picascia S., Bassi V., Barba P., Del Pozzo G., Gianfrani C., HLA-DQ2.5 genes    associated with celiac disease risk are preferentially expressed with respect to non-predisposing HLA genes: Implication for anti-gluten T cell response. Journal of Autoimmunity 2016 Jun;70:63-72.

 doi: 10.1016/j.jaut.2016.03.016

https://pubmed.ncbi.nlm.nih.gov/27083396/

Current Position

• 2020 - Research Scientist and scientific supervisor of  Flow cytometry facility at IGB-ABT, CNR, Naples, Italy

Research Training

• 2018-2020 Post-doc at  Immunonutrition lab of Prof.Roberto Berni Canani, dipartimento di Scienze Mediche e Traslazionali , Università di Napoli Federico II
• 2017-2018 Researcher at IRBM Science Park
• 2007-2017 co-responsible of  Flow cytometry facility at IGB-ABT, CNR, Naples, Italy
• 2010-2017 Post doc at IGB-ABT, CNR, Naples, Italy
• 2007-2010 PhD student at IGB-ABT, CNR, Naples, Italy
• 2006 -2007 Fellow at Regional Center for Education (GEAR), University of Naples “Federico II”, Napoli, Italy
• 2005-2006 Fellow at IGB-ABT
• Undergraduate student at IGB-ABT, CNR, Naples, Italy

Education

• 2015 admission of admission to the national register of expert in cytometry, sponsored by the Italian Society of Cytometry (GIC)
• 2010 PhD in Advanced biology, University of Naples “Federico II”, Italy
• 2005 B.A. in Biology, University of Naples “Federico II”, Italy

Funded Research Projects

• Fondazione Italiana Celiachia 2021